Magneto-Controlled Enzyme Activity with Locally Produced pH Changes.

J Phys Chem Lett

Department of Chemistry and Biomolecular Science, Clarkson University, 8 Clarkson Avenue, Potsdam, New York 13699, United States.

Published: March 2021

AI Article Synopsis

  • The study explores the behavior of amyloglucosidase (AMG) when it is attached to superparamagnetic nanoparticles, which can be controlled by a magnetic field.
  • Applying this magnetic field causes the nanoparticles to clump together or separate, affecting the performance of AMG.
  • This process leads to local changes in pH due to the presence of urease or esterase enzymes on the nanoparticles, ultimately altering AMG's enzymatic activity.

Article Abstract

Biocatalytic activity of amyloglucosidase (AMG), immobilized on superparamagnetic nanoparticles, is dynamically and reversibly activated or inhibited by applying a magnetic field. The magnetic field triggers aggregation/deaggregation of magnetic particles that are also functionalized with urease or esterase enzymes. These enzymes produce a local pH change in the vicinity of the particles changing the AMG activity.

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http://dx.doi.org/10.1021/acs.jpclett.1c00036DOI Listing

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