AI Article Synopsis
- Experimental and epidemiological studies indicate that lead exposure causes epigenetic changes, particularly affecting DNA methylation, chromatin structure, and non-coding RNA expression.
- * The study aimed to explore how the expression of hsa-miR-148a influences DNA methylation status in 85 lead-exposed workers by measuring blood levels and miRNA expression.
- * Results showed an inverse relationship between miR-148a expression and DNA methylation levels, suggesting that higher miR-148a is linked to lower DNA methylation in individuals exposed to lead.
Article Abstract
Experimental and epidemiologic studies have shown that lead (Pb) is able to induce epigenetic modifications, such as changes in DNA methylation profiles, in chromatin remodeling, as well as the expression of non-coding RNAs (ncRNAs). However, very little is known about the interactions between microRNAs (miRNAs) expression and DNA methylation status in individuals exposed to the metal. The aim of the present study was to investigate the impact of hsa-miR-148a expression on DNA methylation status, in 85 workers exposed to Pb. Blood and plasma lead levels (BLL and PLL, respectively) were determined by ICP-MS; expression of the miRNA-148a was quantified by RT-qPCR (TaqMan assay) and assessment of the global DNA methylation profile (by measurement of 5-methylcytosine; % 5-mC) was performed by ELISA. An inverse association was seen between miR-148a and % 5-mC DNA, as a function of BLL and PLL (β = -3.7; = 0.071 and β = -4.1; = 0.049, respectively) adjusted for age, BMI, smoking, and alcohol consumption. Taken together, our study provides further evidence concerning the interactions between DNA methylation profile and miR-148a, in individuals exposed to Pb.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928366 | PMC |
| http://dx.doi.org/10.3389/fgene.2021.620744 | DOI Listing |
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