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CRISPR/Cas9-Based Lateral Flow and Fluorescence Diagnostics. | LitMetric

CRISPR/Cas9-Based Lateral Flow and Fluorescence Diagnostics.

Bioengineering (Basel)

Department of Pediatrics, Division of Blood and Marrow Transplant & Cellular Therapy, University of Minnesota Medical School, Minneapolis, MN 55455, USA.

Published: February 2021

AI Article Synopsis

Article Abstract

Clustered regularly interspaced short palindromic repeat (CRISPR/Cas) proteins can be designed to bind specified DNA and RNA sequences and hold great promise for the accurate detection of nucleic acids for diagnostics. We integrated commercially available reagents into a CRISPR/Cas9-based lateral flow assay that can detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequences with single-base specificity. This approach requires minimal equipment and represents a simplified platform for field-based deployment. We also developed a rapid, multiplex fluorescence CRISPR/Cas9 nuclease cleavage assay capable of detecting and differentiating SARS-CoV-2, influenza A and B, and respiratory syncytial virus in a single reaction. Our findings provide proof-of-principle for CRISPR/Cas9 point-of-care diagnosis as well as a scalable fluorescent platform for identifying respiratory viral pathogens with overlapping symptomology.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7918862PMC
http://dx.doi.org/10.3390/bioengineering8020023DOI Listing

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