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Osteoclastogenic Potential of Tissue-Engineered Periosteal Sheet: Effects of Culture Media on the Ability to Recruit Osteoclast Precursors. | LitMetric

AI Article Synopsis

  • Cell culture media significantly affect the properties of human osteogenic periosteal sheets used for bone reconstruction.
  • A comparison was made between two media: the conventional Medium 199 and the stem cell medium, MesenPRO, concerning their ability to support osteoclast formation and recruit specific immune cells.
  • Results indicated that the stem cell medium not only promoted greater osteoclast formation but also demonstrated a superior functional capacity for preparing tissue-engineered periosteal sheets.

Article Abstract

Cell culture media influence the characteristics of human osteogenic periosteal sheets. We have previously found that a stem cell medium facilitates growth and collagen matrix formation in vitro and osteogenesis in vivo. However, it has not yet been demonstrated which culture medium is superior for osteoclastogenesis, a prerequisite for reconstruction of normal bone metabolic basis. To address this question, we compared chemotaxis and osteoclastogenesis in tissue-engineered periosteal sheets (TPSs) prepared with two types of culture media. Periosteal tissues obtained from adult volunteers were expanded with the conventional Medium 199 or with the stem cell medium, MesenPRO. Hematopoietic enhanced-green-fluorescent-protein (EGFP)-nude mice were prepared by γ-irradiation of Balb/c nu/nu mice and subsequent transplantation of bone marrow cells from CAG-EGFP C57BL/6 mice. TPSs were implanted subcutaneously into the chimeric mice and retrieved after intervals for immunohistopathological examination. EGFP cells were similarly recruited to the implantation site in both the TPSs prepared, whereas the distribution of CD11b cells was significantly lower in the TPS prepared with the stem cell medium. Instead, osteoclastogenesis was higher in the TPS prepared with the stem cell medium than in the one prepared with the conventional medium. These findings suggest that the stem cell medium is preferable for the preparation of more functional TPSs.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926432PMC
http://dx.doi.org/10.3390/ijms22042169DOI Listing

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