AI Article Synopsis

  • - The study developed a head and neck multicellular tumor spheroid model to evaluate NIR fluorescence-guided surgery cameras' effectiveness in visualizing resection margins.
  • - FaDu spheroids were treated with indocyanine green (ICG) and embedded in a tissue-like phantom to test the Fluobeam® NIR camera; results showed significant fluorescence intensity at specific ICG concentrations and spheroid sizes.
  • - The findings suggest that a configuration of 450 μm FaDu spheroids treated with 0.05 mg/ml ICG for 24 hours offers optimal stability and signal intensity, providing a feasible model for comparing NIR camera performance.

Article Abstract

Near-infrared (NIR) fluorescence-guided surgery is an innovative technique for the real-time visualization of resection margins. The aim of this study was to develop a head and neck multicellular tumor spheroid model and to explore the possibilities offered by it for the evaluation of cameras for NIR fluorescence-guided surgery protocols. FaDu spheroids were incubated with indocyanine green (ICG) and then included in a tissue-like phantom. To assess the capability of Fluobeam® NIR camera to detect ICG in tissues, FaDu spheroids exposed to ICG were embedded in 2, 5 or 8 mm of tissue-like phantom. The fluorescence signal was significantly higher between 2, 5 and 8 mm of depth for spheroids treated with more than 5 µg/mL ICG ( < 0.05). The fluorescence intensity positively correlated with the size of spheroids ( < 0.01), while the correlation with depth in the tissue-like phantom was strongly negative ( < 0.001). This multicellular spheroid model embedded in a tissue-like phantom seems to be a simple and reproducible in vitro tumor model, allowing a comparison of NIR cameras. The ideal configuration seems to be 450 μm FaDu spheroids incubated for 24 hours with 0.05 mg/ml of ICG, ensuring the best stability, toxicity, incorporation and signal intensity.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922741PMC
http://dx.doi.org/10.3390/ijms22041966DOI Listing

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