[A simple and fast enzyme immunoassay for the quantitative determination of C-reactive protein].

J Clin Chem Clin Biochem

Staatliches Institut für Immunpräparate und Nährmedien, Berlin, DDR.

Published: February 1988

AI Article Synopsis

  • The method uses enzyme-labelled CRP and serum CRP competing for antibodies attached to a surface, allowing for measurement of CRP levels.
  • The detection limit of the test is 10 micrograms per liter, with a precision of 2.9% near the normal/pathological threshold of 5 mg/l.
  • The test can be completed in just 2 hours.

Article Abstract

The principle is based on the competitive binding of the enzyme-labelled CRP (enzyme: alkaline phosphatase) and the serum CRP to solid phase-bound antibodies. The detection limit was 10 micrograms/l. The precision in the series in the border-line "normal" to "pathological" (5 mg/l) was 2.9%; the precision from day to day at the concentration of 5 mg/l was 4.5%. Only 2 h are required for the whole test.

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