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Development of qPCR Detection Assay for Potato Pathogen Based on a Unique Target Sequence. | LitMetric
The recent taxonomic diversification of bacterial genera and , which cause soft rot in plants, focuses attention on the need for improvement of existing methods for the detection and differentiation of these phytopathogens. This research presents a whole genome-based approach to the selection of marker sequences unique to particular species of . The quantitative real-time PCR assay developed is selective in the context of all tested strains and is able to detect fewer than 10 copies of target DNA per reaction. The presence of plant DNA extract did not affect the sensitivity of the assay.
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