Express Arabidopsis Cryptochrome in Sf9 Insect Cells Using the Baculovirus Expression System.

Methods Mol Biol

National Key Laboratory of Plant Molecular Genetics (NKLPMG), CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences, Shanghai, P. R. China.

Published: June 2021

The Bac-to-Bac Baculovirus Expression System provides a rapid and efficient method to generate recombinant cryptochrome (CRY) proteins with chromophore flavin (FAD), which showed blue light response in vitro.

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Source
http://dx.doi.org/10.1007/978-1-0716-1370-2_16DOI Listing

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