Ancient fermented food has been studied mainly based on residue analysis and recipes and reconstruction attempts were performed using modern domesticated yeast. Furthermore, microorganisms which participated in fermentation were studied using ancient-DNA techniques. In a recent paper, we presented a novel approach based on the hypothesis that enriched yeast populations in fermented beverages could have become the dominant species in storage vessels and their descendants could be isolated and studied today. Here we present a pipeline for isolation of yeast from clay vessels uncovered in archeological sites and transferred to the microbiology lab where they can be isolated and characterized. This method opens new avenues for experimental archeology and enables attempts to recreate ancient food and beverages using the original microorganisms.
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http://dx.doi.org/10.21769/BioProtoc.3473 | DOI Listing |
J Vis Exp
December 2024
Department of Biomedical Engineering, Columbia University;
Vascular organoids derived from human induced pluripotent stem cells (hiPSCs) recapitulate the cell type diversity and complex architecture of human vascular networks. This three-dimensional (3D) model holds substantial potential for vascular pathology modeling and in vitro drug screening. Despite recent advances, a key technical challenge remains in reproducibly generating organoids with consistent quality, which is crucial for downstream assays and applications.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
Cancer Hospital of Dalian University of Technology, State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China. Electronic address:
Currently, vascular grafting is the preferred option to replace or bypass the defective vascular segments, but finding materials with good biocompatibility and diversity alternative for practical clinical applications are still the challenge. The construction of tissue engineered blood vessels (TEBVs) with complex structures will be realized using 3D bioprinting technology, which provides a new idea for vascular transplantation. In this paper, the decellularized extracellular matrix (dECM)/nano clay (NC)/sodium alginate (SA) hybrid bioink was prepared to construct tubular scaffolds in vitro by coaxial 3D bioprinting.
View Article and Find Full Text PDFColloids Surf B Biointerfaces
March 2025
School of Nano-Tech and Nano-Bionics, University of Science and Technology of China (USTC), Hefei 230026, PR China; CAS Key Laboratory for Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics (SINANO), Chinese Academy of Sciences, Suzhou 215123, PR China. Electronic address:
Sci Rep
October 2024
Neutron Spectroscopy Department, HUN-REN Centre for Energy Research, Budapest, Hungary.
Within archaeological studies of ancient pottery, understanding the techniques used to form vessels from unfired clay provides significant information on the history of technology and economic systems, as well as wider cultural practices and social interactions. We introduce here a new analytical methodology, using small-angle neutron scattering (SANS) to investigate pottery forming techniques through the preferential orientation of nanoscale objects within pottery fabrics. Significantly, SANS is non-destructive, suitable for both coarse and fine-textured pottery fabrics, provides quantitative data, enables fast-throughput of samples, and is not significantly affected by surface modifications occurring after the primary forming stage.
View Article and Find Full Text PDFToxics
September 2024
College of Environmental Science and Engineering, Dalian Maritime University, Dalian 116026, China.
Oily sludge (OS) has long been regarded as a hazardous waste, and improper disposal may lead to serious environmental concerns and human health risks. Despite various methods having been proposed and applied to the treatment of OS, the oil occurrence states and properties in sludge are rarely characterized, which may directly link to the selection and effectiveness of treatment methods. Here, confocal laser scanning microscopy (CLSM), X-ray diffraction (XRD), gas chromatography (GC), and four components (SARA) analysis were utilized to characterize the changes in the oil occurrence states and compositions in OS samples before and after high-speed stirring (HSS) treatment.
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