Recognition of (Sesc) for Easy Identification of and Molecular and Phenotypic Study of Β-Lactam Resistance in Isolates in Isfahan.

Background: Not only is it crucial to rapidly detect isolates from a broad range of bacteria, but recognizing resistance agents can greatly improve current diagnostic and therapeutic strategies.

Methods: The current cross-sectional study investigated 120 clinical isolates from a nosocomial infection. The isolates were identified using common biochemical tests, and specific surface protein C () primers were used to confirm the presence of . PCR and special primers were used to detect the β-lactamase gene (). Methicillin resistance was measured using the agar screening method and antibiotic susceptibility was measured by disk diffusion.

Results: 100 samples were characterized as using a phenotypic and genotypic methods. From the 100 specimens examined, 80% contained . According to agar screening, 60% of isolates were methicillin-resistant. isolates demonstrated the highest resistance to penicillin (93%) and the highest sensitivity to cefazolin (39%).

Conclusion: The increased resistance to β-lactam antibiotics in isolates is alarming, and certain precautions should be taken by healthcare systems to continuously monitor the antimicrobial pattern of , so that an appropriate drug treatment can be established.