Molecular Detection of Campylobacter Species: Comparision of with , and Sequencing.

Background: spp. are the main cause of human gastroenteritis. The sequencing is one of fast molecualr method to detect this fastidious. In this study, we compared the sequencing of genewith four housekeeping genestodetect spp. in patients with diarrhea and healthy people.

Methods: 60 samples of DNA extracted from stool samples of 30 patients with diarrhea and 30 healthy people were used. In order to detect , we designed primers for proliferation of , and genes using Primer 3, Mega 4.0 and Blast software. Then the PCR products were sequenced using ABI system.

Results: The sequencing showed concordance of PCR-products with deposited sequences in the Gene Bank. Among diarrhea patients, 53.3% of samples were significantly (p< 0.05) positive for and genes and 50% of samples were positive using , and genes by PCR assay. The average of sensitivity and specificity were found 53.33% and 83.33%, respectively.

Conclusion: Due to various copies of repeated sequences of gene, analyzing its amplicons on electrophoresis may be more difficult than the and genes. According to our results, among the 5 studied genes; the highest detection rate was related to and genes. Although, and genes,instead of gene, can be considered as appropriate genes for molecular detection of bacteria.