AI Article Synopsis
- Nucleic acid detection techniques are crucial for accurate diagnostics in various fields but can be affected by carryover contamination, leading to false positives.
- Effective methods like sodium hypochlorite solution and PCRguard reagent were tested and found to successfully eliminate nucleic acid contamination from liquids and surfaces.
- The combination of these two solutions is recommended for preventing both carryover contamination and aerosol-related issues in molecular diagnostic labs.
Article Abstract
Nucleic acid detection technique has good sensitivity and specificity and is widely used in in vitro diagnosis, animal and plant commodity quarantine, forensic identification, and other fields. However, it is susceptible to carryover contamination during the operation and leads to false-positive results, which seriously affects the detection accuracy. Therefore, finding an effective solution to prevent and eliminate nucleic acid carryover contamination has become particularly urgent. This study compared several different methods for removing nucleic acid contamination and confirmed that sodium hypochlorite solution and PCRguard reagent could effectively eliminate nucleic acid carryover in the liquid and on surfaces of different materials. Besides, the combination of sodium hypochlorite solution and PCRguard can solve the nucleic acid aerosol contamination. This study proposes solutions for the routine prevention of carryover contamination and removal of aerosol that has occurred in molecular diagnostic laboratories.
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| http://dx.doi.org/10.13345/j.cjb.200390 | DOI Listing |
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