AI Article Synopsis
- Human embryonic stem cells have significant potential in regenerative medicine due to their ability to self-renew and differentiate into multiple cell types.
- The study explores a modified culture medium, ActA-mTeSR, replacing TGFβ with Activin A to better preserve the cells' pluripotent state.
- Results indicate that cells grown in ActA-mTeSR express key pluripotency factors, maintain their genetic integrity, and can still differentiate into various cell types, making this an important tool for further research on stem cell biology.
Article Abstract
Human embryonic stem cells exhibit great potential as a therapeutic tool in regenerative medicine due to their self-renewal and trilineage differentiation capacity. Maintaining this unique cellular state has been shown to rely primarily on the Activin A / TGFβ signaling pathway. While most conventional culture media are supplemented with TGFβ, in the current study we utilize a modified version of the commercially available mTeSR1, substituting TGFβ for Activin A in order to preserve pluripotency. (1) Cells cultured in ActA-mTesR express pluripotency factors NANOG, OCT4 and SOX2 at comparable levels with cells cultured in TGFβ-mTeSR. (2) ActA-mTeSR cultured cells retain a physiological karyotype. (3) Cells in ActA-mTeSR maintain their trilineage differentiation capacity as shown in the teratoma formation assay. This system can be used to dissect the role of Activin A, downstream effectors and signaling cascades in human embryonic stem cell responses.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893420 | PMC |
| http://dx.doi.org/10.1016/j.dib.2021.106844 | DOI Listing |
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