AI Article Synopsis
- The balance between osteoblasts and marrow adipocytes is crucial for bone health, and aging can lead to a shift favoring adipocyte formation, resulting in osteoporosis.
- DANCR plays a significant role in regulating the differentiation of bone marrow mesenchymal stem cells (BM-MSCs) into osteoblasts, with its overexpression hindering this process by affecting specific gene markers.
- The interaction between DANCR, miR-1301-3p, and PROX1 forms a pivotal regulatory axis that influences the osteogenic differentiation of human BM-MSCs, providing insights into potential therapeutic targets for bone health.
Article Abstract
The balance of osteoblasts and marrow adipocytes from bone marrow mesenchymal stem cells (BM-MSCs) maintains bone health. Under aging or other pathological stimuli, BM-MSCs will preferentially differentiate into marrow adipocytes and reduce osteoblasts, leading to osteoporosis. Long non-coding RNA differentiation antagonizing non-protein coding RNA (DANCR) participates in the osteogenic differentiation of human BM-MSCs, but the mechanism by which DANCR regulates the osteogenic differentiation of human BM-MSCs has not been fully explained. We observed that DANCR and prospero homeobox 1 (PROX1) were downregulated during osteogenic differentiation of human BM-MSCs, while miR-1301-3p had an opposite trend. DANCR overexpression decreased the levels of alkaline phosphatase, RUNX2, osteocalcin, Osterix in BM-MSCs after osteogenic induction, but DANCR silencing had the opposite result. Moreover, DANCR sponged miR-1301-3p to regulate PROX1 expression. miR-1301-3p overexpression reversed the suppressive role of DANCR elevation on the osteogenic differentiation of human BM-MSCs. Also, PROX1 elevation abolished the promoting role of miR-1301-3p overexpression on the osteogenic differentiation of human BM-MSCs. In conclusion, DANCR suppressed the osteogenic differentiation of human BM-MSCs through the miR-1301-3p/PROX1 axis, offering a novel mechanism by which DANCR is responsible for the osteogenic differentiation of human BM-MSCs.
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| http://dx.doi.org/10.1007/s11010-021-04074-9 | DOI Listing |
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