Transcriptional Profile of Virulence Genes in Patients with Gastritis and Gastric Cancer.

Introduction: Numerous molecular epidemiology studies have been performed about the frequency of virulence genes in patients with infection so far. This study was conducted to detect transcriptional profile by cDNA of virulence genes in gastric biopsy samples of gastritis and gastric carcinoma patients.

Materials And Methods: In a case-control study, based on the prevalence of gastritis and gastric cancer in Sanandaj city during 2018 and 2019, 23 and 11 gastric antral biopsy samples with infection were collected from gastritis and gastric carcinoma patients by the consecutive and available sampling method. Pathological characters, including tumor grades and tumor areas for gastric carcinoma biopsy samples prepared from gastric cancer areas, were determined by the pathologist. Total RNA of gastric antral biopsy samples was extracted, and their cDNA was synthesized by TaKaRa kit. virulence genes' cDNA using specific primers and PCR was detected. This study's results were analyzed by SPSS version 25 and statics chi-square tests for determination of relationship and correlation between cDNAs of transcriptional profile and clinical outcomes infection, including gastritis, gastric carcinoma, tumor grades, and tumor area.

Results: The positive statistical correlations were observed between transcripts of , , and genes and infection clinical outcomes ( < 0.05).

Conclusion: Detection of the virulence genes' cDNA in gastric biopsy samples can help provide the prognosis of clinical outcomes.