AI Article Synopsis
- The study focuses on the molecular epidemiology of Carbapenem-resistant Klebsiella pneumoniae (CR-KP) isolates, particularly those producing New Delhi metallo-β-lactamase (NDM), highlighting its global public health threat.
- Out of 100 CR-KP isolates collected from a Lahore hospital, 90 were found to be metallo-β-lactamase (MBL) producers, with 75 specifically identified as NDM producers, including subtypes NDM-1 and NDM-5.
- The majority of NDM-positive isolates showed complete resistance to β-lactam antibiotics but remained susceptible to colistin, with a significant proportion carrying specific plasmids linked to antibiotic resistance.
Article Abstract
Carbapenem-resistant (CR-KP) particularly New Delhi metallo-β-lactamase (NDM) is a serious public health concern globally. The aim of the study to determine the molecular epidemiology of -producing clinically isolated . Carbapenem-resistant isolates (n = 100) were collected from tertiary care hospital Lahore. Isolates were confirmed by VITEK 2 system and MALDI-TOF. Minimum inhibitory concentration was performed by VITEK 2 and molecular characterization was done by PCR, PFGE, DNA hybridization and replicon typing. Of 90 MBL-producing , 75 were NDM producers; 60 were NDM-1 and 11 NDM-5. A total of 27 belonged to ST11 and 14 to ST147. NDM-positive isolates were 100% resistant to β-lactam antibiotics except for colistin. 13.3% isolates carried on ∼140 kb plasmids. A total of 32 (52.4%) isolates were positive for IncA/C and 18 (29.5%) IncF/II. The extensively resistant lineage of NDM-producing is prevalent in the clinical setting.
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| http://dx.doi.org/10.2217/fmb-2020-0315 | DOI Listing |
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