Induction of abortion in mice with a monoclonal antibody specific for suppressor T-lymphocyte molecules.

A monoclonal antibody, mAb 14-30, which binds T-cell produced suppressor factors (TsF) was used to study the possibility that molecules produced by suppressor T-cells play a role in maintaining pregnancy, presumably by protecting the fetus from the maternal immune system. Female mice were injected with mAb 14-30 at various times after mating. Overall, only 14% of the expected 68% of the mated and treated females were pregnant at term. In addition, Western blots were used to demonstrate the presence of TsF in fetuses, placentae, uteri and spleen of pregnant animals and its presence only in the spleen of non-pregnant animals. These experiments help to confirm results that indicate the importance of immune suppressor factors in maintaining pregnancy and extend these previous observations to include suppressor T-cell molecules.