AI Article Synopsis
- The study aims to determine how luteolin affects apoptosis, cell cycle progression, and the expression/secretion of VEGF in human choroidal melanoma cells (C918 and OCM-1).
- Various concentrations of luteolin were tested, and significant changes in cell growth and apoptosis rates were observed through different experimental methods like flow cytometry and Western blot analysis.
- Results indicated that luteolin treatment led to decreased cell fusion, increased apoptosis, cell cycle arrest, and reduced expression of VEGF and Bcl-2, while Bax expression increased, suggesting luteolin's potential as a therapeutic agent in melanoma.
Article Abstract
Aim: To investigate the effects of luteolin on apoptosis, the cell cycle, and the expression and secretion of vascular endothelial growth factor (VEGF) in human choroidal melanoma cells (C918 and OCM-1).
Methods: C918 and OCM-1 cells cultured were treated with various concentrations of luteolin (0, 5, 10, 15 µmol/L). Cell growth was observed with an inverted microscope, and cell cycle arrest was detected by propidium iodide (PI) staining using flow cytometry. Apoptosis was detected by Hoechst33342 staining, and apoptosis rate was determined by Annexin V-FITC/PI experiments using flow cytometry. The expression of apoptosis-related proteins Bcl-2, Bax and VEGF was analyzed using Western blots. The levels of VEGF secreted by the cells into the supernatant was analyzed using ELISA.
Results: After treating with 5 to 15 µmol/L luteolin for 48h, the fusion degree of C918 and OCM-1 cells decreased, and more floating apoptotic cells appeared. Luteolin treatment increased the G0-G1 phase ratio of the C918 and OCM-1 cells, blocked cell cycle progression, and increased the apoptosis rate of the C918 and OCM-1 cells. Western blot showed that luteolin decreased the expression of Bcl-2 and VEGF in the C918 and OCM-1 cells and increased the expression of Bax protein. The ELISA results showed that 10 to 15 µmol/L luteolin decreased the cell secretion of VEGF.
Conclusion: Luteolin may induce apoptosis by regulating the levels of apoptosis-related proteins in C918 and OCM-1 cells. Luteolin can induce cell cycle arrest, decrease the expression of VEGF.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840359 | PMC |
| http://dx.doi.org/10.18240/ijo.2021.02.02 | DOI Listing |
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- Results indicated that luteolin treatment led to decreased cell fusion, increased apoptosis, cell cycle arrest, and reduced expression of VEGF and Bcl-2, while Bax expression increased, suggesting luteolin's potential as a therapeutic agent in melanoma.
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