This paper presents a microfluidic device capable of performing genetic analysis on dung samples to identify White Rhinoceros (). The development of a microfluidic device, which can be used in the field, offers a portable and cost-effective solution for DNA analysis and species identification to aid conservation efforts. Optimization of the DNA extraction processes produced equivalent yields compared to conventional kit-based methods within just 5 minutes. The use of a color-changing loop-mediated isothermal amplification reaction for simultaneous detection of the cytochrome B sequence of enabled positive results to be obtained within as little as 30 minutes. Field testing was performed at Knowsley Safari to demonstrate real-world applicability of the microfluidic device for testing of biological samples.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882957 | PMC |
| http://dx.doi.org/10.1002/ece3.7053 | DOI Listing |
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