Antibiofilm Activity of Small-Molecule ZY-214-4 Against .

is the most important pathogenic bacteria in humans. As the resistance of to existing antibiotics is increasing, there is an urgent need for new anti-infective drugs. biofilms cause persistent infections and resist complete eradication with antibiotic therapy. The present study investigated the inhibitory effect of the novel small-molecule ZY-214-4 (C H BrNO) on biofilm formation. At a subinhibitory concentration (4 μg/ml), ZY-214-4 had no effect on the growth of strains and also showed no cytotoxicity in human normal bronchial epithelial cells (Bease-2B). The results of a semi-quantitative biofilm test showed that ZY-214-4 prevented biofilm formation, which was confirmed by scanning electron microscopy and confocal laser scanning microscopy. ZY-214-4 significantly suppressed the production of polysaccharide intercellular adhesion and prevented cell aggregation, and also inhibited the mRNA expression of and other biofilm-related genes (, , , , , α, and β) in clinical isolates. Thus, at a subinhibitory concentration, ZY-214-4 inhibits biofilm formation by preventing cell aggregation, highlighting its clinical potential for preventing or treating infections.