AI Article Synopsis
- Histamine poisoning from seafood poses a major public health risk, prompting the development of a new detection method.
- A competitive sandwich immunoassay using a modified biotin-streptavidin system was created, achieving an impressive correlation coefficient of 0.9942 and a broad detection range for histamine.
- This new method, requiring no sample derivatization, demonstrated high recovery rates in seafood samples and a lower detection limit compared to traditional testing methods, making it suitable for regular seafood safety checks.
Article Abstract
Histamine poisoning from seafood is a significant public health and safety concern. To detect histamine sensitively and accurately, a novel competitive sandwich immunoassay using a modified biotin-streptavidin system coupling with polylysine was developed. Using this strategy, a sandwich ELISA with an IC value of 112.8 ng mL and a broad linear range of 11.7-1500 ng mL with a correlation coefficient of 0.9942 was validated. Without any sample derivatization procedure, the recovery of histamine ranged from 80.19% to 108.3% with a coefficient of variation of 1.43-11.7% in tuna, prawn and crab. The sandwich ELISA had a detectionlimit of 5.86 ng mL, which was 15-fold lower than an indirect competitive ELISA (ic-ELISA). This simple, sensitive and accurate method can be applied to detect histamine in routine seafood samples.
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| http://dx.doi.org/10.1016/j.foodchem.2021.129196 | DOI Listing |
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