Cryptosporidiosis has been identified as a significant underlying cause of morbidity and mortality worldwide. Studies in high and low income countries have recognized the importance of Cryptosporidium as a cause of diarrhea. The objectives of the current study were to determine the prevalence rate and genotypes of Cryptosporidiumin in diarrheic children in Makkah Region. A total of 1,380 fecal samples were collected from children aged up to 14 years attending 3 major hospitals of Makkah between March 2015 and March 2016. Stool collected were subjected to direct microscopic examination and crypto antigen detection using ImmunoCard STAT, Cryptosporidium/Giardia rapid test. Part of each positive stool sample was kept frozen at -20ºC for molecular characterization. Initial screening by immunochromatographic detection kit revealed 23 positive cases. PCR was performed for positive cases by amplification of a piece of the gene encoding the small (18S) subunit of rRNA producing a 435-438 bp product. Cryptosporidium genotyping was performed by RFLP analysis of PCR products. Genotyping revealed 18 cases C. hominis genotype, 4 cases C. parvaum genotype and one sample failed to be amplified. The data revealed a higher incidence of the common human species C. hominis (81.8%). The detection of both C. hominis and C. parvaum genotypes point to the possibility of both anthroponotic and zoonotic transmission routes occurring in Makkah region. Further studies are needed to verify the subgenotypes of Cryptosporidium to elucidate the real transmission modes and hence plan for effective control strategies.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Burden of rotavirus and adenovirus gastroenteritis in children and adults hospitalized in two geo-climatically different provinces of Sri Lanka.
Virusdisease
December 2024
Department of Statistics and Computer Science, Faculty of Science, University of Kelaniya, Kelaniya, 11600 Sri Lanka.
Acute gastroenteritis is common in infants and children of Sri Lanka. There is limited information on the burden of rotavirus gastroenteritis in infants and children in Sri Lanka but none in adults. Adenovirus gastroenteritis is not previously reported in Sri Lanka.
View Article and Find Full Text PDFGut microbiome profile to the level species in diarrheic protozoan-carrier patients in Italy.
Life Sci
December 2024
Biomedical Research Center, Qatar University, Doha, Qatar.
The human gastrointestinal microbiota contains a diverse consortium of microbes, including bacteria, protozoa, viruses, and fungi that are involved in many physiological and metabolic as well pathogenetic processes. However, microbiological research is dominated by studies describing the impact of prokaryotic bacteria on gut microbiome with a limited understanding of their relationship with other integral microbiota constituents as protozoa. Here, we investigated the gut microbiome composition using Oxford Nanopore Technology approach in relation to protozoan colonization of Giardia duodenalis, Cryptosporidium parvum, Blastocystis sp.
View Article and Find Full Text PDFOccurrence and diversity of Campylobacter species in diarrheic children and their exposure environments in Ethiopia.
PLOS Glob Public Health
October 2024
National Food Institute, Technical University of Denmark, Lyngby, Denmark.
Campylobacter is a major zoonotic foodborne pathogen that poses a significant public health threat, particularly among children and immunocompromised individuals. However, data on the occurrence and sources of Campylobacter infection remain scarce in Ethiopia. This study assessed the occurrence, diversity, and relationships between Campylobacter from diarrheic children and potential exposure sources using whole-genome sequencing.
View Article and Find Full Text PDFEmergence of non-classical genotype constellations of G9P[8] rotavirus strains in diarrheic children in Sabah, Malaysia.
Infect Genet Evol
November 2024
Borneo Medical and Health Research Centre, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, 88400 Kota Kinabalu, Sabah, Malaysia; Department of Pathology and Microbiology, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, 88400 Kota Kinabalu, Sabah, Malaysia. Electronic address:
Article Synopsis
- - The study focuses on the G9P[8] rotavirus A genotype, which has been the main type found in Malaysia since the 2000s, and aims to explore its genetic characteristics and evolution.
- - Researchers analyzed the complete genomes of three G9P[8] strains from young children in Sabah, discovering that they show a DS-1-like genotype instead of the typical Wa-like arrangement.
- - Phylogenetic analysis indicated that two strains were genetically similar to G9P[8]-E2 strains from Japan, hinting at a shared ancestry, and the findings are essential for improving rotavirus monitoring and developing vaccines.
Molecular characterization of common zoonotic protozoan parasites and bacteria causing diarrhea in dairy calves in Ningxia Hui Autonomous Region, China.
Parasite
October 2024
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China.
Article Synopsis
- Diarrhea caused by zoonotic pathogens is a major health concern for dairy calves and poses risks to humans, especially children.
- A study conducted on 343 fecal samples from calves in China found a 91% positive rate for various pathogens, with *Clostridium perfringens* being the most prevalent at 61.5%.
- Coinfections were common, with 67.3% of samples containing two to five different pathogens, and the research identified significant variations in pathogenic presence related to farm, age, and season.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!