imaging of a PVD neuron in .

The nematode nociceptive PVD neurons have highly ordered dendritic branches, making this an ideal model to study the development and organization of dendrites. A -promoter-driven GFP reporter line [GFP] provides a comprehensive visualization of PVD anatomy. Here, we describe the detailed procedures for imaging a PVD neuron using at late L4 larval stage by confocal microscopy. This protocol can also be applied to imaging other cells in . For complete details on the use and execution of this protocol, please refer to Feng et al. (2020).