Molecular identification of Gnathostoma spinigerum (Nematoda: Gnathostomatidae) as causative agent of human gnathostomiasis in Thailand.

A 43-year-old male residing in Prachin Buri Province, Thailand, was admitted to the Siriraj University Hospital of Mahidol University, Thailand, in July 2014 with right eyelid swelling and serpiginous lesion for three weeks. A nematode specimen was accidentally recovered from his upper right eyelid area. The body of the worm was cylindrical and measured 11.0 × 1.4 mm. The head bulb had eight circles of transverse spines. Anterior half of the body was covered with rows of comb-like pointed spines. The tail part was rounded dorsally and flattened ventrally and no copulatory spicule was observed at the posterior end. It was morphologically identified as an immature female worm of gnathostome species. Sequence analysis for partial cytochrome c oxidase subunit I (COI) gene revealed this recovered nematode as Gnathostoma spinigerum.