AI Article Synopsis
- Two in-house RNA extraction protocols for SARS-CoV-2 detection were tested, utilizing magnetic bead extraction with automated systems.
- The performance of these protocols was compared to a commercial kit, with no significant differences in detection capability, though the commercial kit was slightly more efficient.
- Using in-house methods resulted in substantial cost savings, allowing labs to save between €350 and €400 per 96 samples extracted compared to commercial options.
Article Abstract
Background: Two automatable in-house protocols for high-troughput RNA extraction from nasopharyngeal swabs for SARS-CoV-2 detection have been evaluated.
Methods: One hundred forty one SARS-CoV-2 positive samples were collected during a period of 10-days. In-house protocols were based on extraction with magnetic beads and designed to be used with either the Opentrons OT-2 (OT-2in-house) liquid handling robot or the MagMAXTM Express-96 system (MMin-house). Both protocols were tested in parallel with a commercial kit that uses the MagMAXTM system (MMkit). Nucleic acid extraction efficiencies were calculated from a SARS-CoV-2 DNA positive control.
Results: No significant differences were found between both in-house protocols and the commercial kit in their performance to detect positive samples. The MMkit was the most efficient although the MMin-house presented, in average, lower Cts than the other two. In-house protocols allowed to save between 350€ and 400€ for every 96 extracted samples compared to the commercial kit.
Conclusion: The protocols described harness the use of easily available reagents and an open-source liquid handling system and are suitable for SARS-CoV-2 detection in high throughput facilities.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886139 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0246302 | PLOS |
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