AI Article Synopsis
- The study focused on using a polyethylene glycol-dextran two-phase polymer system to effectively isolate exosome-enriched extracellular nanovesicles (ENVs) from human plasma.
- Systematic testing was conducted to find the best combination of polymer molecular mass and concentration for optimal phase separation, followed by in-depth analysis using techniques like nanoparticle tracking and Raman spectroscopy.
- The isolated ENVs were characterized and their protein and microRNA contents evaluated, showcasing a promising new method for isolating plasma ENVs for potential diagnostic applications.
Article Abstract
The aim of the study was to explore the polyethylene glycol-dextran two-phase polymer system formed in human plasma to isolate the exosome-enriched fraction of plasma extracellular nanovesicles (ENVs). Systematic analysis was performed to determine the optimal combination of the polymer mixture parameters (molecular mass and concentration) that resulted in phase separation. The separated phases were analyzed by nanoparticle tracking analysis and Raman spectroscopy. The isolated vesicles were characterized by atomic force microscopy and dot blotting. In conclusion, the protein and microRNA contents of the isolated ENVs were assayed by flow cytometry and by reverse transcription followed by quantitative polymerase chain reaction (RT-qPCR), respectively. The presented results revealed the applicability of a new method for plasma ENV isolation and further analysis with a diagnostic purpose.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867002 | PMC |
| http://dx.doi.org/10.3390/polym13030458 | DOI Listing |
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