To explore the relationship of oxidative stress and TGF-β 1/Smad3 pathway in the inhibition of osteoblast mineralization by copper chloride (CuCl), the osteoblasts were treated with CuCl (0, 50 μM, 100 μM, 150 μM CuCl 5HO) for 24 h. We found that Cu impaired the osteoblast structure, inhibited the glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities, alkaline phosphatase (ALP) content, mRNA expression of collagen I (COL-I), osteocalcin (OCN), insulin-like growth factor I (IGF-I), bone morphogenetic protein-2 (BMP-2), transforming growth factor β1 (TGF-β1) and core-binding factor α1 (Cbfα1), promoted the reactive oxygen species (ROS) production, inactivated the TGF-β1/Smad3 pathway. It indicates that the inactivated TGF-β1/Smad3 pathway leads to osteoblast impairment by CuCl. It will contribute to clarify the influence of CuCl on the osteoblast mineralization.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.etap.2021.103613 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of bone metabolism on hematopoiesis: A Mendelian randomization study.
Osteoporos Sarcopenia
December 2024
Department of Pharmacology and Pharmacy, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong.
Objectives: Osteoblast is known to regulate hematopoiesis according to preclinical studies but the causal relationship in human remains uncertain. We aimed to evaluate causal relationships of bone mineral density (BMD) with blood cell traits using genetic data.
Methods: Summary statistics from the largest available genome-wide association study were retrieved for total body BMD (TBBMD), lumbar spine BMD (LSBMD), femoral neck BMD (FNBMD) and 29 blood cell traits including red blood cell, white blood cell and platelet-related traits.
Efficacy of the Bushen Jianpi Huoxue Formula on Beclin-1/Bcl-2-mediated autophagy and apoptosis in osteoblasts.
Front Pharmacol
January 2025
The Third Clinical Medical School, Guangzhou University of Chinese Medicine, Guangzhou, China.
Background: The Beclin-1/Bcl-2 complex plays a pivotal role in regulating both autophagy and apoptosis in osteoblasts affected by osteoporosis. This study first investigates whether the Bushen Jianpi Huoxue Formula can enhance the cellular function of osteoblasts. Additionally, it initially explores the functional mechanism of Beclin-1/Bcl-2-related apoptosis.
View Article and Find Full Text PDFPTPA localized in the Golgi apparatus plays an important role in osteoblast differentiation.
Biochem Biophys Res Commun
January 2025
Department of Oral Morphology, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, Okayama, Japan. Electronic address:
Regulatory subunits of protein phosphatase 2A (PP2A) define the substrate and functional specificity of the PP2A holoenzyme within specific organelles. While PP2A regulates osteoblast differentiation, the roles and localization of its regulatory subunits in osteoblasts remain unclear. Here, we identified PTPA, a PP2A regulatory protein, predominantly localized to the Golgi apparatus, closely overlapping with the Golgi marker Giantin.
View Article and Find Full Text PDFThe Impact of a Human Bone Morphogenetic Protein-infused Nano-Bone Material Combined With Traditional Chinese Medicine Carthamus tinctorius on the Repair of Oral Bone Defects.
Int Dent J
January 2025
Department of Stomatology, Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing, 100091, China. Electronic address:
Background: The role of human bone morphogenetic protein-infused in combination with traditional Chinese medicine Carthamus tinctorius in repairing oral bone defects (OBD) was evaluated in this work.
Methods: An experiment was conducted on a rat OBD model, where rats were randomly rolled into Groups A, B, and C based on their different treatments. Micro-CT was utilized to measure jaw bone volume fraction (BV/TV) and bone mineral density (BMD) in rats, while a biomechanical testing machine assessed compressive strength of the jaw bone.
Plasma-electrolytic oxidation: A rapid single step post processing approach for additively manufactured biomedical implants.
Biomater Adv
January 2025
School of Chemical Engineering, University of Birmingham, Edgbaston B15 2TT, United Kingdom. Electronic address:
Laser-powder bed fusion (PBF-LB) has enabled production of customised skeletal implants that incorporate porous lattices structures to enable bone ingrowth. However, the inherent surface roughness of PBF-LB, characterised by partially adhered particles and undulating sub-topography, remains a barrier to adoption. As such PBF-LB surfaces require several time-consuming post-processing steps, nevertheless, conventional finishing techniques are often limited by geometrical part complexity, making them unsuitable for porous PBF-LB parts.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!