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Use of Simvastatin, Fibrin Clots, and Their Combination to Improve Human Ovarian Tissue Grafting for Fertility Restoration After Anti-Cancer Therapy. | LitMetric

AI Article Synopsis

  • Chemotherapy can damage ovaries and lead to the loss of ovarian follicles, limiting fertility options for cancer survivors; one potential solution is the pre-chemotherapy preservation of ovarian tissue.* -
  • Past attempts to transplant frozen ovarian tissue have seen live births, but significant follicle loss occurs due to slow blood vessel reformation in the graft, highlighting the need for improved techniques to enhance vascularization.* -
  • The study explored using simvastatin and embedding tissue in fibrin clots to improve grafting success, finding that certain treatments led to higher follicle survival and vascularization, indicating promising strategies for ovarian restoration in cancer survivors.*

Article Abstract

Anticancer treatments, particularly chemotherapy, induce ovarian damage and loss of ovarian follicles. There are limited options for fertility restoration, one of which is pre-chemotherapy cryopreservation of ovarian tissue. Transplantation of frozen-thawed human ovarian tissue from cancer survivors has resulted in live-births. There is extensive follicular loss immediately after grafting, probably due to too slow graft revascularization. To avoid this problem, it is important to develop methods to improve ovarian tissue neovascularization. The study's purpose was to investigate if treatment of murine hosts with simvastatin or/and embedding human ovarian tissue within fibrin clots can improve human ovarian tissue grafting (simvastatin and fibrin clots promote vascularization). There was a significantly higher number of follicles in group A (ungrafted control) than in group B (untreated tissue). Group C (simvastatin-treated hosts) had the highest levels of follicle atresia. Group C had significantly more proliferating follicles (Ki67-stained) than groups B and E (simvastatin-treated hosts and tissue embedded within fibrin clots), group D (tissue embedded within fibrin clots) had significantly more proliferating follicles (Ki67-stained) than group B. On immunofluorescence study, only groups D and E showed vascular structures that expressed both human and murine markers (mouse-specific platelet endothelial cell adhesion molecule, PECAM, and human-specific von Willebrand factor, vWF). Peripheral human vWF expression was significantly higher in group E than group B. Diffuse human vWF expression was significantly higher in groups A and E than groups B and C. When grafts were not embedded in fibrin, there was a significant loss of human vWF expression compared to groups A and E. This protocol may be tested to improve ovarian implantation in cancer survivors.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862713PMC
http://dx.doi.org/10.3389/fonc.2020.598026DOI Listing

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