SDS is widely used in sample preparation for proteomic research. However, SDS is incompatible with LC and electrospray ionization. SDS depletion is therefore required ahead of LC-MS analysis. Most of current SDS removal strategies are time consuming, laborious, and have low reproducibility. Here, we describe a method, SDS-cyclodextrin (CD)-assisted sample preparation, by which CD can bind to SDS and form CD-SDS complexes in solutions, allowing for direct tryptic digestion. We demonstrate that SDS-CD-assisted sample preparation is a simple, fast, and robust SDS-based sample preparation method for proteomics application.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7970136 | PMC |
| http://dx.doi.org/10.1016/j.mcpro.2021.100051 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Target-regulated AgS/FeOOH heterojunction activity: a direct label-free photoelectrochemical immunosensor.
Mikrochim Acta
January 2025
College of Geography and Environmental Sciences, College of Chemistry and Materials Science, Zhejiang Normal University, Jinhua, 321004, China.
Myoglobin (Mb), an important cardiac marker, plays a crucial role in diagnosing, monitoring, and evaluating the condition of patients with cardiovascular diseases. Here, we propose a label-free photoelectrochemical (PEC) sensor for the detection of Mb through target regulated the photoactivity of AgS/FeOOH heterojunction. The AgS/FeOOH nanospindles were synthesized and served as a sensing platform for the fabrication of bio-recognized process for Mb.
View Article and Find Full Text PDFInfluence of the Type of Bulk-Fill, Resin-Based Composite on Internal Adaptation in Class V Restorations.
J Coll Physicians Surg Pak
January 2025
Department of Stomatology, The Second People's Hospital of Hefei and Hefei Hospital Affiliated to Anhui Medical University, Hefei, Anhui, China.
Objective: To investigate the effects of bulk-fill, resin-based composite types (high or low viscosity) on the internal adaptation of Class V restorations.
Study Design: Experimental study. Place and Duration of the Study: Hefei Stomatological Hospital, Hefei, China, from October 2022 to December 2023.
A SPR aptamer sensor for mercury based on AuNPs@NaYF:Yb,Tm,Gd upconversion luminescent nanoparticles.
Anal Methods
November 2017
Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
A new aptamer-based surface plasmon resonance (SPR) system has been designed to detect Hg that utilizes near-infrared (NIR)-to-NIR gold nanoparticle coated NaYF:Yb,Tm,Gd up-conversion nanoparticles (AuNPs@NaYF:Yb,Tm,Gd UCNPs) as probes. The AuNPs@NaYF:Yb,Tm,Gd UCNPs were prepared and excited by near-infrared light (980 nm) which emitted at a near-infrared wavelength (808 nm) using an inexpensive infrared continuous wave laser diode. The AuNPs@NaYF:Yb,Tm,Gd UCNPs were conjugated with Hg aptamers.
View Article and Find Full Text PDFPreparation of a novel RAM-MIP for selective solid-phase extraction and gas chromatography determination of heptachlor, endosulfan and their metabolite residues in pork.
Anal Methods
November 2017
College of Environmental and Chemical Engineering, Nanchang Hangkong University, Nanchang 330063, China.
A novel method was established using a restricted access material combined with a molecularly imprinted polymer (RAM-MIP) as the sorbent material in solid phase extraction (SPE) for clean-up of α-endosulfan, β-endosulfan, endosulfate, endosulfan-ether, endosulfan lactone, heptachlor, heptachlor--epoxide, and heptachlor--epoxide in pork and gas chromatography (GC) for determination. The RAM-MIP was prepared by precipitation polymerization by using endosulfan as the template, methacrylic acid (MAA) as the monomer, glycidyl methacrylate (GMA) as the pro-hydrophilic co-monomer, ethylene glycol dimethacrylate (EGDMA) as the crosslinker, azobisisobutyronitrile (AIBN) as the initiator, and toluene as the porogen. Ultraviolet spectroscopy (UV) and H-nuclear magnetic resonance (H-NMR) analysis verified that MAA interacted specifically with endosulfan in a ratio of 1 : 1 in the pre-polymerization solution.
View Article and Find Full Text PDFDetermining the required data elements to develop the information management system for Iranian traditional medicine.
BMC Complement Med Ther
January 2025
Department of Health Information Technology, Abadan University of Medical Sciences, Abadan, Iran.
Background: Currently, there is no agreed-upon data collection tool for comprehensively structured documentation of Iranian traditional medicine (ITM) from the information management perspective. As ITM practice varies significantly from current medicine in diagnosis and treatment approaches, it is not appropriate to use data platforms or information systems developed for current medicine. Consequently, the collected data are non-comparable, reducing the verdicts' generalization.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!