This article reviews the scientific literature on electromembrane extraction (EME) of peptides and amino acids. In EME, target analytes are extracted from aqueous sample, through a supported liquid membrane (organic) and into a microliter volume of aqueous buffer (acceptor). Experimental conditions and performance for EME of peptides and amino acids are reviewed and discussed in detail, providing readers with an overview and basic understanding of the subject. In addition, this review discuss the potential for future applications, and scientific questions that need to be addressed for EME of peptides and amino acids to be generally accepted. EME is under commercialization, and therefore we expect it will be an active area of research in the near future.

Download full-text PDF

Source
http://dx.doi.org/10.4155/bio-2020-0285DOI Listing

Publication Analysis

Top Keywords

peptides amino
16
amino acids
16
eme peptides
12
electromembrane extraction
8
eme
5
peptides
4
extraction peptides
4
amino
4
acids
4
acids status
4

Similar Publications

Recent studies have suggested that the interaction between diet and an individual's genetic predisposition can determine the likelihood of obesity and various metabolic disorders. The current study aimed to examine the association of dietary branched-chain amino acids(BCAAs) and aromatic amino acids(AAAs) with the expression of the leptin and FTO genes in the visceral and subcutaneous adipose tissues of individuals undergoing surgery. This cross-sectional study was conducted on 136 Iranian adults, both men and women, aged ≥18 years.

View Article and Find Full Text PDF

Hyperuricemia, a disorder of purine metabolism associated with cardiovascular disease, gout, and kidney disease, can be alleviated by food-derived peptides. However, the precise mechanisms remain unclear, hindering their development. This study reviews uric acid-lowering peptides from various sources, focusing on two pathways: inhibiting uric acid production and promoting excretion.

View Article and Find Full Text PDF

Whey proteins have anti-fatigue activity, but there are few studies that have reported the ameliorative effects of branched-chain amino acid (BCAA) oligopeptides from whey proteins on fatigue in mice. The purposes of this study were to establish a process for the preparation of BCAA oligopeptides from whey protein and to investigate the anti-fatigue activity of BCAA oligopeptides. Whey proteins were hydrolyzed by trypsin and flavourzyme and purified by ethanol precipitation and reversed-phase high performance liquid chromatography (RP-HPLC).

View Article and Find Full Text PDF

JAG1/Notch Pathway Inhibition Induces Ferroptosis and Promotes Cataractogenesis.

Int J Mol Sci

January 2025

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou 510060, China.

Cataracts remain the leading cause of visual impairment worldwide, yet the underlying molecular mechanisms, particularly in age-related cataracts (ARCs), are not fully understood. The Notch signaling pathway, known for its critical role in various degenerative diseases, may also contribute to ARC pathogenesis, although its specific involvement is unclear. This study investigates the role of Notch signaling in regulating ferroptosis in lens epithelial cells (LECs) and its impact on ARC progression.

View Article and Find Full Text PDF

Structural Characterization and Immune Activation Capacity of Peptidoglycan from in RAW264.7 Cells.

Int J Mol Sci

December 2024

Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Risk Assessment Laboratory of Animal Product Quality Safety Feed Source Factors of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research of Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Peptidoglycan (PGN) is a unique component of prokaryotic cell walls with immune-enhancing capacities. Here, we extracted PGN from , a by-product of amino acid fermentation, using the trichloroacetic acid (TCA) method. SDS-PAGE analysis confirmed the presence of PGN, with a band of approximately 28 kDa.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!