We have previously adapted a select number of cell lines to grow in serum-free media supplemented with fly extract. This condition is arguably more representative of a native growth environment. Here, we validated that the fly extract adapted line, S2R+ (FEx 2.5%) is amenable to RNAi. RNAi against in both S2R+ and S2R+ (FEx 2.5%) produced phenotypes similar to ones previously described in S2 cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7846936 | PMC |
| http://dx.doi.org/10.17912/micropub.biology.000362 | DOI Listing |
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