Disease causing bacteria such as and present a problem for fish farming. Treatment to remove them are generally carried out using antibiotics which have side effects on fish, the environment and humans. However, the use of antibacterial compounds derived from heterotrophic bacteria serve as a good alternative for antibiotics. Therefore, this study aimed to explore antibacterial activity in the secondary metabolite extracts of heterotrophic bacteria against and . : Heterotrophic bacteria namely JS04 MT102913.1, JS08 MT102920.1, JS10 MT102922.1, sp. JS11 MT102923.1, sp. JS19 MT102924.1, JS22 MT102926.1, and sp. strain JS25 MT102927.1 were used in this study. The sequences of these bacteria have been deposited and are available from NCBI GenBank. Each heterotrophic bacterium was cultured on 6L nutrient broth for 8 days, and extracts produced using ethyl acetate to obtain their secondary metabolites. These extracts were tested for their phytochemical contents using FT-IR and also tested for their inhibitory property in pathogenic bacteria by agar diffusion method. Phytochemical test results showed that the seven heterotrophic bacterial isolates produced terpenoid compounds. Based on the inhibitory test, the secondary metabolite extracts from sp strain JS04 had the highest inhibitory effect on the growth of pathogenic bacteria namely, (17.5 mm), (16.8 mm), and (17.3 mm). : It was concluded that the secondary metabolite extracts of heterotrophic bacteria inhibit the growth of and .
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http://dx.doi.org/10.12688/f1000research.26215.1 | DOI Listing |
Mar Drugs
December 2024
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
SR21, a kind of eukaryotic heterotrophic organism rich in unsaturated fatty acids, is an emerging microbial alternative to fish oil. The dietary inclusion of 15% SR21 was optimal for the growth performance of zebrafish. Previous studies demonstrated that fructose-1,6-bisphosphate aldolase (FBA) of is a valuable broad-spectrum antigen against various pathogens in aquaculture (e.
View Article and Find Full Text PDFGlob Chang Biol
December 2024
Stockbridge School of Agriculture, University of Massachusetts Amherst, Amherst, Massachusetts, USA.
Anthropogenic nitrogen (N) deposition is unequally distributed across space and time, with inputs to terrestrial ecosystems impacted by industry regulations and variations in human activity. Soil carbon (C) content normally controls the fraction of mineralized N that is nitrified (ƒ), affecting N bioavailability for plants and microbes. However, it is unknown whether N deposition has modified the relationships among soil C, net N mineralization, and net nitrification.
View Article and Find Full Text PDFJ Biol Chem
December 2024
Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling Rd 189, Qingdao 266101, China; Shandong Energy Institute, Songling Rd 189, Qingdao 266101, China; Qingdao New Energy Shandong Laboratory, Songling Rd 189, Qingdao 266101, China.
2-O-α-Glucosylglycerol (GG) is a natural heteroside synthesized by many cyanobacteria and a few heterotrophic bacteria under salt stress conditions. Bacteria produce GG in response to stimuli and degrade it once the stimulus diminishes. Heterotrophic bacteria utilize GG phosphorylase (GGP), a member of the GH13_18 family, via a two-step process consisting of phosphorolysis and hydrolysis for GG catabolism.
View Article and Find Full Text PDFBioresour Technol
December 2024
Department of Ecology, College of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong, China. Electronic address:
This study cultivated a bacterial consortium (S60) from landfill leachate that exhibited effective heterotrophic nitrification and aerobic denitrification (HN-AD) properties. Under aerobic conditions, the removal of NH-N reached 100 % when the S60 consortium utilised NH-N either as the sole nitrogen source or in combination with NO-N and NO-N. Optimal HN-AD performance was achieved with sodium acetate as a carbon source and a pH of 7.
View Article and Find Full Text PDFMethodsX
December 2024
Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu Darul Iman, Malaysia.
Determining the optimum application dosage of probiotic in biofloc system is often challenging because the microbial community seems to exert similar effects irrespective of their dosages. It is however noted that certain dosages promote higher yield in shrimp culture more effectively. Principal component analysis was adopted to identify these optimum dosages where 1-way ANOVA could not clearly identify due to the effects of microbial community.
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