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http://dx.doi.org/10.1164/rccm.202012-4445LE | DOI Listing |
J Virol
January 2025
Microbiology and Immunology, Carver College of Medicine, The University of Iowa, Iowa City, Iowa, USA.
Measles virus (MeV) is a highly contagious respiratory virus transmitted via aerosols. To understand how MeV exits the airways of an infected host, we use unpassaged primary cultures of human airway epithelial cells (HAE). MeV typically remains cell-associated in HAE and forms foci of infection, termed infectious centers, by directly spreading cell-to-cell.
View Article and Find Full Text PDFHeart Lung
January 2025
Programa de Pós-Graduação em Ciências Pneumológicas, Universidade Federal do Rio Grande do Sul - Rua Ramiro Barcelos, 2350, Sala 2050, CEP 90035-003, Porto Alegre, RS, Brazil; Unidade de Fisiologia Pulmonar, Hospital de Clínicas de Porto Alegre - Rua Ramiro Barcelos, 2350, Sala 2050, CEP 90035-003, Porto Alegre, RS, Brazil.
Background: Pulmonary function testing (PFT) is paramount in assessing patients with respiratory symptoms and chronic cardiopulmonary diseases. Although seminal studies have demonstrated that PFT generates aerosols, this simple observation does not confirm the potential for enhanced pathogen transmission.
Objective: We aimed to describe the frequency of patients who developed suspected symptoms of COVID-19, prompting SARS-CoV-2 testing after undergoing PFT during the reopening of a laboratory amid the deceleration of the pandemic.
Sci Rep
January 2025
Gunma University, 1-5-1 Tenjin-Cho, Kiryu, 376-8515, Japan.
With the emergence of COVID-19 variants and new viruses, it remains uncertain when the next pandemic will occur. A lockdown is considered the last resort to halt the spread of infection; however, it causes significant economic and social damage. Therefore, exploring less harmful alternatives during such scenarios is crucial.
View Article and Find Full Text PDFSci Adv
January 2025
Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.
Pathogenic bioaerosols are critical for outbreaks of airborne disease; however, rapidly and accurately identifying pathogens directly from complex air environments remains highly challenging. We present an advanced method that combines open-set deep learning (OSDL) with single-cell Raman spectroscopy to identify pathogens in real-world air containing diverse unknown indigenous bacteria that cannot be fully included in training sets. To test and further enhance identification, we constructed the Raman datasets of aerosolized bacteria.
View Article and Find Full Text PDFMicroorganisms
December 2024
Department of Environmental Science, University of Arizona, Tucson, AZ 85719, USA.
Resuspended particles from human activities can contribute to pathogen exposure via airborne fomite contamination in built environments. Studies investigating the dissemination of resuspended viruses are limited. The goal of this study was to explore viral dissemination after aerosolized resuspension via human activities on indoor flooring.
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