sp. nov., isolated from cornfield soil.

Strain L22-9, a Gram-stain-negative and rod-shaped bacterium, motile by one polar flagellum, was isolated from cornfield soil in Bijie, Guizhou Province, PR China. Based on 16S rRNA gene sequences, it was identified as a species. Multilocus sequence analysis of concatenated 16S rRNA, , and gene sequences showed that strain L22-9 formed a clearly separated branch, located in a cluster together with LMG 21623, DSM 13647 and DSM 13194. Whole-genome comparisons based on average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) confirmed that strain L22-9 should be classified as a novel species. It was most closely related to DSM 13647 with ANI and dDDH values of 91.87 and 46.3 %, respectively. Phenotypic features that can distinguish strain L22-9 from DSM 13647 are the assimilation ability of -acetyl-d-glucosamine, poor activity of arginine dihydrolase and failure to ferment ribose and d-fucose. The predominant cellular fatty acids of strain L22-9 are C, summed feature 3 (C ω6 and/or C ω7) and summed feature 8 (C ω7 and/or C ω6). The respiratory quinones consist of Q-9 and Q-8. The polar lipids are diphosphatidylglycerol, phosphatidylethanolamine, two unidentified phosphoglycolipids, two unidentified aminophospholipids and an unidentified glycolipid. Based on the evidence, we conclude that strain L22-9 represents a novel species, for which the name sp. nov. is proposed. The type strain is L22-9 (=CGMCC 1.18528=LMG 31948), with a DNA G+C content of 60.85 mol%.