Kaempferol ameliorates the regulatory effects of / on epithelial-mesenchymal transition through the PAK4/β-catenin axis in SRA01/04 cells.

To investigate whether kaempferol exhibits a protective effect on high glucose-induced epithelial-mesenchymal transition (EMT) by mediating the / and PAK4/β-catenin pathways in SRA01/04 cells. qRT-PCR and western blot assays were used for gene and protein determination, and migration and invasion assays were conducted. A coimmunoprecipitation assay was used for determining protein interactions. High glucose effectively upregulated expression, downregulated expression and promoted cell migration and invasion. Kaempferol attenuated high glucose-induced EMT by increasing expression and decreasing expression. was identified as a direct target of . overexpression could rescue the effects of deficiency on SRA01/04 cells. Kaempferol ameliorated the regulatory effects of / on high glucose-induced EMT through PAK4/β-catenin in SRA01/04 cells.