AI Article Synopsis
- * PCR testing is being used more in labs for detecting MAP in feces, proving to be fast and reliable compared to traditional fecal cultures, although extracting DNA from fecal samples can be tricky.
- * A study tested a specific nucleic acid extraction method combined with a gene PCR and found it accurately identified all test samples for both positive and negative results, demonstrating its effectiveness for diagnosing MAP in cattle.
Article Abstract
Johne's disease (paratuberculosis) is an economically important disease of cattle worldwide. The disease is caused by subsp. (MAP), a fastidious gram-positive bacterium. PCR is increasingly used in diagnostic laboratories for the detection of MAP in fecal samples given the rapid test turnaround time and sensitivity and specificity comparable to fecal culture. However, efficient extraction of DNA for sensitive detection of MAP by PCR is affected by the complex lipid-rich cell wall of MAP and the presence of PCR inhibitors in feces. We evaluated a high-throughput nucleic acid extraction method (MagMAX core nucleic acid purification kit with mechanical lysis module) in conjunction with an gene PCR for the detection of MAP from bovine fecal samples, which resulted in correct identification of all negative (13 of 13) and positive (35 of 35) proficiency test samples obtained from the National Veterinary Services Laboratories. In addition, all 6 negative and 50 of 51 positive diagnostic specimens tested were categorized correctly.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953105 | PMC |
| http://dx.doi.org/10.1177/1040638721991118 | DOI Listing |
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