Most outbreaks of Shiga toxin-producing (STEC) are attributed to consumption of contaminated foodstuffs including beef and dairy products. In this study, we evaluated the prevalence of non-O157 STEC cultured from beef and dairy cattle and collected in Xinjiang Uygur Autonomous Region in China. Results identified 67 non-O157 STEC recovered from the 793 samples including beef cattle (10.28%, 43/418) and dairy cattle (6.40%, 24/375). A total of 67 non-O157 STEC was sequenced allowing for analyses of their serotypes, virulence genes, and identification of the corresponding multilocus sequence types (STs). Twenty-one O serogroups and nine H serotypes were identified and the dominant serotype identified was O22:H8. One subtype (a) and four subtypes (, , , and ) were found in the 67 non-O157 STEC isolates. The results revealed that +-positive STEC isolates were predominant (32.83%, 22/67), followed by + (29.85%, 20/67) and alone (17.91%, 12/67). Non-O157 STEC isolates carried virulence genes (98.51%), (53.73%), and (17.91%). Of the four adherence-associated genes tested, was absent, whereas and were present in 67 and 55 non-O157 STEC isolates, respectively. The STEC isolates were divided into 48 pulsed-field gel electrophoresis patterns and 10 STs, and ST446 (O22:H8) was the dominant clone (22.38%). Our results revealed that there was a high genetic diversity among non-O157 STEC isolated from beef and dairy cattle, some of which have potential to cause human diseases.
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