Previous research reported that KLF3 plays different roles in the regulation of adipose deposition across species. However, the exact function of KLF3 in goat subcutaneous adipocyte remains unknown. Here, the goat KLF3 gene was firstly cloned and showed that the mRNA sequence of the goat KLF3 gene was 1,264 bp (GenBank accession number: KU041753.1) and its coding sequence was 1,037 bp, encoding 345 amino acids with three classic zinc finger domains of KLFs family at its C-terminus. The alignment of the amino acid sequence of KLF3 among various species demonstrated that goat had the highest homology to that of sheep, presenting 99.4% similarity, while the homology similarity to that of mice presented only 93.62% in contrast. Furthermore, KLF3 had highest mRNA level in fat tissue and lowest level in the heart in comparison. Additionally, the mRNA level of KLF3 gradually tended to increase during adipogenesis. Interestingly, overexpression of KLF3 increased lipid accumulation. In line with this, the gain-of-function of KLF3 dramatically elevated the mRNA levels of TG synthetic genes and adipogenic maker genes (p < .01) . Moreover, overexpression of KLF3 upregulated all the potential target genes, except for C/EBPα. These results suggested that KLF3 is a positive regulator for subcutaneous adipocyte differentiation in goats.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/asj.13514 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Single-Cell Multiomics Reveals TCR Clonotype-Specific Phenotype and Stemness Heterogeneity of T-ALL Cells.
Cell Prolif
December 2024
Department of Systems Biomedical Sciences, School of Medicine, Jinan University, Guangzhou, China.
T-cell acute lymphoblastic leukaemia (T-ALL) is a heterogeneous malignant disease with high relapse and mortality rates. To characterise the multiomics features of T-ALL, we conducted integrative analyses using single-cell RNA, TCR and chromatin accessibility sequencing on pre- and post-treatment peripheral blood and bone marrow samples of the same patients. We found that there is transcriptional rewiring of gene regulatory networks in T-ALL cells.
View Article and Find Full Text PDFProtective Effect of IgY Embedded in W/O/W Emulsion on LPS Enteritis-Induced Colonic Injury in Mice.
Nutrients
October 2024
Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, Ministry of Education, China Agricultural University, Beijing 100083, China.
Article Synopsis
- Chicken yolk immunoglobulin (IgY) serves as a natural alternative to antibiotics for treating enteritis and was tested in a special emulsion to enhance its effectiveness.
- In the study, various emulsifiers were used, and the one with whey protein isolate (WPI) proved to be the most effective in protecting mice from intestinal damage caused by LPS.
- The findings indicated that IgY in the emulsion significantly improved intestinal health by reducing inflammation, enhancing antioxidant properties, and protecting intestinal tissue integrity.
Alterations of Krüppel-like Factor Signaling and Potential Targeted Therapy for Hepatocellular Carcinoma.
Anticancer Agents Med Chem
September 2024
Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, & Department of Medical Immunology, Medical School of Nantong University, Nantong 226001, Jiangsu Province, China.
Krüppel-like factors (KLFs, total 18 members) from the zinc finger protein (ZFP) super-family have a wide range of biological functions in hepatocellular carcinoma (HCC). This paper reviews the recent some progresses of aberrant KLFs with their potential values for diagnosis, prognosis, and targeted therapy in HCC. The recent advances of oncogenic KLFs in the diagnosis, prognosis, and targeted therapy of HCC were reviewed based on the related literature on PUBMED and clinical investigation.
View Article and Find Full Text PDFIL23R mutations associated with decreased risk of psoriasis lead to the differential expression of genes implicated in the disease.
Exp Dermatol
September 2024
Manchester Academic Health Science Centre, Faculty of Biology Medicine and Health, School of Biological Sciences, The University of Manchester, Manchester, UK.
Article Synopsis
- * Researchers developed models using T cells to analyze how different forms of IL23R (wild type vs. protective mutant) impact the signaling pathway via STAT3, identifying genes related to psoriasis risk through RNA sequencing.
- * The study discovered several key genes (like HLA-B, SOCS1, and CCR5) that are differentially regulated based on IL23R form, contributing to a better understanding of the IL23 pathway and its role in psoriasis.
Genome-Wide Identification, Evolution, and miRNA-22 Regulation of () Gene Family in Chicken ().
Animals (Basel)
September 2024
Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan 528225, China.
Article Synopsis
- - The study examines Krüppel-like factors (KLFs), a group of transcription factors found in various species, focusing on their gene family in chickens by analyzing conserved gene sequences from different animals.
- - Several bioinformatics methods were used to analyze the chicken genome, leading to insights about the evolutionary relationships, structural characteristics, and functions of KLFs, particularly noting that birds' KLFs are more similar to mammals and amphibians than to fish.
- - Additionally, the research explored the role of miRNA-22 in influencing the expression of KLFs in the liver, heart, and muscle of Qingyuan partridge chickens, revealing that both interference and overexpression of this miRNA can affect gene expression levels.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!