[Establishment the method for polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans determination in human serum].

Objective: To establish the determination method for polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans(PCDD/Fs) in human serum, and further to provide an operable and scientific determination protocal and basis for conducting human health risk assessment for PCDD/Fs.

Methods: The serum samples were pretreated by C18 column solid phase extraction, acid silica gel column and activated carbon column purification, separated by DB-5 MS capillary column(60 m×0. 25 mm×0. 25 μm), and PCDD/Fs was quantitative analyzed by high resolution mass spectrometry.

Results: The method detection limit was in the range of 0. 35-3. 26 pg/g lipid. This method was further validated using international serum standard reference material sample SRM 1958. According to the reference mass fraction values given for SRM 1958, the concentrations of 17 PCDD/Fs monomers were all in the range of reference mass fraction values, and the relative standard deviation was 2%-19%(n=3). This method was further applied to determination PCDD/Fs in actual serum of human body. The result showed that the recovery rate of isotope labeled PCDD/Fs internal standards were in the range of 61%-135%.

Conclusion: The performance of the method is highly sensitive, stable and highly accurate, which meets the requirements for the determination of PCDD/Fs in human serum and the method can be applied to human health risk assessment for PCDD/Fs in the future.