AI Article Synopsis
- Hepcidin, produced by the liver, is an antimicrobial peptide that regulates iron balance and has potential cytotoxic effects on cancer cells.
- Research investigated hepcidin's impact on myeloma cells using MTT and DNA fragmentation assays, revealing that it impairs cell survival and causes DNA damage.
- The study concluded that hepcidin can lyse myeloma cells, indicating a possible new role in anticancer immunity beyond its known functions.
Article Abstract
Background/aim: Hepcidin is a cationic acute phase reactant synthesized by the liver. It has bactericidal properties and is a major regulator of iron homeostasis. Cationic antimicrobial peptides represent an innate antimicrobial defense system. We hypothesized that, like other cationic antimicrobial peptides, hepcidin is cytotoxic to cancer cells.
Materials And Methods: The cytotoxicity of human hepcidin against myeloma cells was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) and DNA fragmentation assays. Plasma membrane damage was quantified by propidium iodide (PI) staining. Cell membrane changes were visualized by scanning electron microscopy.
Results: Hepcidin impaired myeloma cell survival and induced DNA fragmentation. PI staining and scanning electron microscopy revealed hepcidin-induced disruption of the plasma membrane.
Conclusion: Human hepcidin is an anti-cancer peptide that induces myeloma cell lysis, and therefore may play a role in innate anticancer immunity. To our knowledge, this is the first biological function ascribed to human hepcidin that is not related to its antimicrobial and iron-regulatory properties.
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| http://dx.doi.org/10.21873/anticanres.14811 | DOI Listing |
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