AI Article Synopsis
- Diosgenin (DGN) is a plant-derived sterol similar to cholesterol, gaining attention for its pharmacological effects.
- The study found that both DGN and cholesterol can similarly affect lipid bilayer membranes, reducing phase transition enthalpy and orienting themselves parallel to the membrane normal.
- However, DGN at 30 mol% changes the interactions of lipid headgroups differently than cholesterol, indicating that while it behaves like cholesterol at lower concentrations, it starts to alter membrane properties significantly at higher levels.
Article Abstract
Diosgenin (DGN), which is a sterol occurring in plants of the Dioscorea family, has attracted increasing attention for its various pharmacological activities. DGN has a structural similarity to cholesterol (Cho). In this study we investigated the effects of the common tetracyclic cores and the different side chains on the physicochemical properties of lipid bilayer membranes. Differential scanning calorimetry showed that DGN and Cho reduce the phase transition enthalpy to a similar extent. In H NMR, deuterated-DGN/Cho and POPC showed similar ordering in POPC bilayers, which revealed that DGN is oriented parallel to the membrane normal like Cho. It was suggested that the affinity of DGN-Cho in membrane is stronger than that of DGN-DGN or Cho-Cho interaction. P NMR of POPC in bilayers revealed that, unlike Cho, DGN altered the interactions of POPC headgroups at 30 mol%. These results suggest that DGN below 30 mol% has similar effects with Cho on basic biomembrane properties.
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| http://dx.doi.org/10.1016/j.bmcl.2021.127816 | DOI Listing |
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