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PFK activation is essential for the odontogenic differentiation of human dental pulp stem cells. | LitMetric

PFK activation is essential for the odontogenic differentiation of human dental pulp stem cells.

Biochem Biophys Res Commun

Department of Health Sciences, The Graduate School of Dong-A University, Busan, 49315, Republic of Korea; Department of Biological Sciences, Dong-A University, Busan, 49315, Republic of Korea. Electronic address:

Published: March 2021

AI Article Synopsis

  • Dental pulp stem cells (DPSCs) can turn into different cell types, including those that help create dentin, which is crucial for repairing dental pulp and regenerating teeth.
  • The study reveals that during the process of differentiating into odontoblasts, DPSCs experience an increase in aerobic glycolysis, primarily driven by specific enzymes (PFKM and PFK2) influenced by AKT activation.
  • The heightened activity of the PFK enzyme is vital for promoting aerobic glycolysis, which is essential in the differentiation of DPSCs into cells that contribute to tooth development and potential pulp repair.

Article Abstract

Dental pulp stem cells (DPSCs) can differentiate into diverse cell lineages, including odontogenic cells that are responsible for dentin formation, which is important in pulp repair and tooth regeneration. While glycolysis plays a central role in various cellular activities in both physiological and pathological conditions, its role and regulation in odontogenic differentiation are unknown. Here, we show that aerobic glycolysis is induced during odontoblastic differentiation from human DPSCs. Importantly, we demonstrate that during odontoblastic differentiation, protein expression levels of phosphofructokinase 1 muscle isoform (PFKM) and PFK2, but not other glycolytic enzymes, are mainly upregulated by AKT activation, resulting in increased total PFK enzyme activity. Increased PFK activity is essential to enhance aerobic glycolysis, which plays an important role in the odontoblastic differentiation of human DPSCs. These findings underscore that PFK activation-induced aerobic glycolysis accompanies, and participates in, human DPSCs differentiation into odontogenic lineage, and could play a role in the regulation of dental pulp repair.

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Source
http://dx.doi.org/10.1016/j.bbrc.2021.01.059DOI Listing

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