A PHP Error was encountered

Severity: Warning

Message: file_get_contents(https://...@gmail.com&api_key=61f08fa0b96a73de8c900d749fcb997acc09): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests

Filename: helpers/my_audit_helper.php

Line Number: 143

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 143
Function: file_get_contents

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 209
Function: simplexml_load_file_from_url

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 994
Function: getPubMedXML

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3134
Function: GetPubMedArticleOutput_2016

File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

Temporally integrated single cell RNA sequencing analysis of PBMC from experimental and natural primary human DENV-1 infections. | LitMetric

AI Article Synopsis

  • Dengue human infection studies help explore key questions in flavivirus biology, but there is limited understanding of the immune response differences between attenuated and wild-type DENV infections.
  • This study used single-cell RNA sequencing (scRNAseq) to compare the transcriptional responses of primary DENV-1 infections from controlled human challenge studies and natural infections.
  • Findings showed that while both infection types activated similar inflammatory responses, natural infections led to stronger suppression of genes related to protein translation and mitochondrial function in monocytes, indicating a deeper anti-viral effect.

Article Abstract

Dengue human infection studies present an opportunity to address many longstanding questions in the field of flavivirus biology. However, limited data are available on how the immunological and transcriptional response elicited by an attenuated challenge virus compares to that associated with a wild-type DENV infection. To determine the kinetic transcriptional signature associated with experimental primary DENV-1 infection and to assess how closely this profile correlates with the transcriptional signature accompanying natural primary DENV-1 infection, we utilized scRNAseq to analyze PBMC from individuals enrolled in a DENV-1 human challenge study and from individuals experiencing a natural primary DENV-1 infection. While both experimental and natural primary DENV-1 infection resulted in overlapping patterns of inflammatory gene upregulation, natural primary DENV-1 infection was accompanied with a more pronounced suppression in gene products associated with protein translation and mitochondrial function, principally in monocytes. This suggests that the immune response elicited by experimental and natural primary DENV infection are similar, but that natural primary DENV-1 infection has a more pronounced impact on basic cellular processes to induce a multi-layered anti-viral state.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7875406PMC
http://dx.doi.org/10.1371/journal.ppat.1009240DOI Listing

Publication Analysis

Top Keywords

natural primary
28
primary denv-1
24
denv-1 infection
24
experimental natural
12
infection
9
primary
8
denv-1
8
response elicited
8
denv infection
8
transcriptional signature
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!