In this study, a series of acidic or alkaline polypeptide chains were designed and grafted onto DEG-AM resin using Fmoc solid-phase synthesis to study the relationship between enzyme conformation and carrier surface charge. β-d-glucosidase (βGase) was then immobilized onto these modified carriers by adsorption. Each form of immobilized Gase showed decreasing specific activity compared to that of the free. It could be attributed to both the changes in the enzyme conformation and the decrease in mass transfer efficiency. The optimum temperature of free Gase, DEG@B3-Gase is 55 °C, which of DEG@A3-Gase is 65 °C and they all have the highest activity at pH 5. The values of free Gase, DEG@A3-Gase, and DEG@B3-Gase are 0.546 kJ/mol, 0.224 kJ/mol, and 0.446 kJ/mol, and the values were 1.30 mmol/L, 1.44 mmol/L and 2.63 mmol/L, respectively. It shows that free Gase and DEG@A3-Gase are more similar. Meanwhile, the free Gase (1.0 g/L, pH 5.0) stored at 4 °C has a shorter half-life (t), which is only 9 days. However, the half-life of DEG@B3-Gase and DEG@A3-Gase is 20 days and over 60 days, indicating that the negative charged surface was conducive to maintenance of the structure and catalytic property of Gase.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/10826068.2021.1875237 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Epitaxy of GaSe Coupled to Graphene: From In Situ Band Engineering to Photon Sensing.
Small
October 2024
School of Physics and Astronomy, University of Nottingham, Nottingham, NG7 2RD, UK.
2D semiconductors can drive advances in quantum science and technologies. However, they should be free of any contamination; also, the crystallographic ordering and coupling of adjacent layers and their electronic properties should be well-controlled, tunable, and scalable. Here, these challenges are addressed by a new approach, which combines molecular beam epitaxy and in situ band engineering in ultra-high vacuum of semiconducting gallium selenide (GaSe) on graphene.
View Article and Find Full Text PDFScalable CIGS Solar Cells Employing a New Device Design of Nontoxic Buffer Layer and Microgrid Electrode.
ACS Appl Mater Interfaces
July 2024
Photovoltaics Research Department, Korea Institute of Energy Research, Daejeon 34129, Republic of Korea.
The efficiency of copper indium gallium selenide (CIGS) solar cells that use transparent conductive oxide (TCO) as the top electrode decreases significantly as the device area increases owing to the poor electrical properties of TCO. Therefore, high-efficiency, large-area CIGS solar cells require the development of a novel top electrode with high transmittance and conductivity. In this study, a microgrid/TCO hybrid electrode is designed to minimize the optical and resistive losses that may occur in the top electrode of a CIGS solar cell.
View Article and Find Full Text PDFCarrier mobility and optical properties of a type-II GaSe/ZnS heterostructure as a photocatalyst: a first-principles study.
Phys Chem Chem Phys
May 2024
National Key Laboratory for Electronic Measurement Technology, North University of China, Taiyuan, 030051, China.
Article Synopsis
- A new van der Waals heterostructure (vdWH) made of GaSe and ZnS was analyzed for its electronic properties and photocatalytic performance, specifically for water splitting.
- The material demonstrated a direct band gap of 2.19 eV, allowing it to effectively absorb visible light, while its type-II band arrangement reduced the recombination of electron-hole pairs.
- Under strong electric fields, the band gap narrowed and shifted the material’s characteristics from semiconductor to metal, enhancing its optical absorption capacity significantly compared to the ZnS monolayer.
Van der Waals Nonlinear Photodetector with Quadratic Photoresponse.
Nano Lett
February 2023
Key Laboratory of Light Field Manipulation and Information Acquisition, Ministry of Industry and Information Technology, and Shaanxi Key Laboratory of Optical Information Technology, School of Physical Science and Technology, Northwestern Polytechnical University, Xi'an710129, China.
With unique electronic and optical attributes and dangling-bond-free surface, two-dimensional (2D) materials have broadened the functionalities of photodetectors. Here, we report a quadratically nonlinear photodetector (QNPD) composed of a van der Waals (vdW) stacked GaSe/InSe heterostructure. Compared with the reported 2D material-based photodetectors, the extra second-harmonic generation (SHG) process in GaSe/InSe leads to the quadratically nonlinear function between photocurrent and optical intensity, extending the photodetection wavelength from 900 to 1750 nm.
View Article and Find Full Text PDFA type-II NGyne/GaSe heterostructure with high carrier mobility and tunable electronic properties for photovoltaic application.
Nanotechnology
November 2022
School of Information Science and Technology, Northwest University, Xi'an, 710127, People's Republic of China.
The two-dimensional heterostructures with type-II band alignment and super-high carrier mobility offer an updated perspective for photovoltaic devices. Here, based on the first-principles calculation, a novel vertical NGyne/GaSe heterostructure with an intrinsic type-II band alignment, super-high carrier mobility (10cmVs), and strong visible to ultraviolet light absorption (10-10cm) is constructed. We investigate the electronic structure and the interfacial properties of the NGyne/GaSe heterostructure under electric field and strain.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!