Enables Cells with the Suspension Cultivation Feature.

Cell-based vaccine manufacturing is an important strategy for viral disease prevention. Cultivating cells in suspension could maximize the utility of large bioreactors for cost-effective and scaled up vaccine production, where adapting adherent cells to suspension culture is the bottleneck and key. Through whole transcriptome sequencing of suspension and adherent strains of BHK-21 and CHO-K1 cells followed by the identification of differentially expressed genes, mutational analysis, gene ontology, and pathway enrichment analysis, we identified four candidate genes, , , , , feasible for genetically modulating anchorage-dependent cells toward cell suspension culture, and experimentally validated the functionality of in both BHK-21 and CHO-K1 cells. Our study unveiled a novel role of that could potentially aid in the establishment of a cost-effective vaccine manufacturing platform relying on cell cultivation in suspension.