Phytochemical investigation on the 80% EtOH extract of the whole plants of afforded three new 4a--plicamine-type alkaloids, zephyranthines A-C (). Structural elucidation of all the compounds were performed by spectral methods such as 1D and 2D (HH COSY, HMQC, and HMBC) NMR spectroscopy, in addition to high resolution mass spectrometry. All the alkaloids were evaluated for their cytotoxic activities against six glioma cell lines (CHG5, SH-SY5Y, SHG44, U251, U343, and GL15). Alkaloids and exhibited significant cytotoxic activities against all tested cell lines with IC values of less than 20 μM.
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