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Community Parameters and Genome-Wide RAD-Seq Loci of Imply Its Transfer between Farmed European Sea Bass and Wild Farm-Aggregating Fish. | LitMetric

Wild fish assemblages that aggregate within commercial marine aquaculture sites for feeding and shelter have been considered as a primary source of pathogenic parasites vectored to farmed fish maintained in net pens at an elevated density. In order to evaluate whether (Isopoda, Cymothoidae), a generalist and pestilent isopod that is frequently found in Adriatic and Greek stocks of farmed European sea bass (), transfers between wild and farmed fish, a RAD-Seq (restriction-site-associated DNA sequencing)-mediated genetic screening approach was employed. The double-digest RAD-Seq of 310 specimens collected from farmed European sea bass (138) and different wild farm-aggregating fish (172) identified 313 robust SNPs that evidenced a close genetic relatedness between the "wild" and "farmed" genotypes. ddRAD-Seq proved to be an effective method for detecting the discrete genetic structuring of and genotype intermixing between two populations. The parasite prevalence in the farmed sea bass was 1.02%, with a mean intensity of 2.0 and mean abundance of 0.02, while in the wild fish, the prevalence was 8.1%; the mean intensity, 1.81; and the mean abundance, 0.15. Such differences are likely a consequence of human interventions during the farmed fish's rearing cycle that, nevertheless, did not affect the transfer of .

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912605PMC
http://dx.doi.org/10.3390/pathogens10020100DOI Listing

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