Purpose: To investigate the effect of application of a primer resembling a universal adhesive with or without light irradiation followed by a hydrophobic bonding agent on bonding effectiveness, based on shear bond strength (SBS) and shear fatigue strength (SFS) tests. Materials and Methods: An experimental two-step self-etch adhesive (BZF) that comprises a primer resembling a universal adhesive (BZP) and a hydrophobic bonding agent (BZB) were used. The two-step self-etch adhesive, Clearfil SE Bond 2 (SE), served as a comparison. Three experiments were conducted. Experiment 1: (1) BZP alone without light irradiation (BZP w/o); (2) BZP alone with light irradiation (BZP w/); (3) BZP without light irradiation followed by BZB (BZPB w/o); (4) BZP with light irradiation followed by BZB (BZPB w/). Experiment 2: (1) BZPB w/o, (2) SE primer + BZ bonding agent (SEP + BZB), and (3) SE primer + SE bonding agent (SEPB). The bonded specimens of experiment 1 and 2 were subjected to SBS tests. Experiment 3: Bonded specimens following the same experimental protocol as experiment 2 were subjected to SFS tests. Results: BZPB showed significantly higher mean enamel and dentin SBS than did BZP. BZPB showed significantly higher SBS without light irradiation than with light irradiation to both substrates. The group of BZPB without light irradiation showed significantly higher SBS than the group of BZPB with light irradiation to both substrates. For experiments 2 and 3, although no significant differences were found in SBS among groups for enamel, SEP + BZB showed a significantly lower SBS and SFS than other groups for dentin. Conclusions: BZF showed bonding performance equivalent to that of the gold standard Clearfil SE Bond 2.

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