Molecular characterization of isolated from hospital acquired sepsis in pediatrics, relation to antibiotics, resistance and virulence genes.

Introduction: The objective of this study was to determine the prevalence of antibiotic resistance genes , , , and virulence genes Panton-Valentine Leucocidin (PVL) and fibronectin-binding protein () among isolates from hospital-acquired sepsis from pediatric intensive care units.

Methods: The study was a retrospective cross-sectional study, including 250 unique isolates of obtained from pediatric patients with hospital-acquired sepsis. The isolates were subjected to study of antibiotic susceptibility by disc diffusion method and molecular analysis of antibiotic resistance genes and certain virulence genes (PVL and genes).

Results: Methicillin resistant represented 178 (71%) of the isolated and reduced susceptibility to vancomycin was detected by minimum inhibitory concentration in 39 (22%) isolates. It was found that there was a strong association between the MRSA strains and resistance to some antibiotics, devices association (p<0.001) and patient outcomes (p=0.003). There was a significant association between reduced vancomycin susceptibility (p=0.010), the presence of a central line catheter (p=0.000) and gene (p<0.001) and mortality rate.

Conclusions: The present study highlights that major strains isolated from sepsis in pediatric patients were methicillin resistant with a substantial proportion of reduced susceptibility to vancomycin. Although none of the isolates had genes responsible for vancomycin resistance, this finding warrants a considerable attention for study as it was a risk factor for mortality in those patients. The virulence genes fibronectin-binding protein and Panton-Valentine Leucocidin were not uncommon in