AI Article Synopsis
- "Nitrosocaldaceae" are a group of microorganisms found in hot springs and geothermally heated soils, playing a crucial role in the nitrogen cycle, but remain largely unstudied due to challenges in isolating them in pure form.
- This study utilized metagenomics to analyze samples from hot springs in India and China, leading to the identification of two new species within "Nitrosocaldus" and a new genus named "Nitrosothermus."
- Key metabolic genes for ammonia oxidation and thermal adaptation were found in the genomes, along with genes for urea degradation and vitamin biosynthesis, highlighting their unique adaptations to high-temperature environments.
Article Abstract
" Nitrosocaldaceae" are globally distributed in neutral or slightly alkaline hot springs and geothermally heated soils. Despite their essential role in the nitrogen cycle in high-temperature ecosystems, they remain poorly understood because they have never been isolated in pure culture, and very few genomes are available. In the present study, a metagenomics approach was employed to obtain " Nitrosocaldaceae" metagenomic-assembled genomes (MAGs) from hot spring samples collected from India and China. Phylogenomic analysis placed these MAGs within " Nitrosocaldaceae." Average nucleotide identity and average amino acid identity analysis suggested the new MAGs represent two novel species of " Nitrosocaldus" and a novel genus, herein proposed as " Nitrosothermus." Key genes responsible for chemolithotrophic ammonia oxidation and a thaumarchaeal 3HP/4HB cycle were detected in all MAGs. Furthermore, genes coding for urea degradation were only present in " Nitrosocaldus," while biosynthesis of the vitamins, biotin, cobalamin, and riboflavin were detected in almost all MAGs. Comparison of ". Nitrosocaldales/Nitrosocaldaceae" with other AOA revealed 526 specific orthogroups. This included genes related to thermal adaptation (cyclic 2,3-diphosphoglycerate, and S-adenosylmethionine decarboxylase), indicating their importance for life at high temperature. In addition, these MAGs acquired genes from members from archaea (Crenarchaeota) and bacteria (Firmicutes), mainly involved in metabolism and stress responses, which might play a role to allow this group to adapt to thermal habitats.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7819960 | PMC |
| http://dx.doi.org/10.3389/fmicb.2020.608832 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Effects of Vegetable Planting Ages on Community Structure of Ammonia-oxidizing Archaea and Ammonia-oxidizing Bacteria in Greenhouse Vegetable Fields].
Huan Jing Ke Xue
October 2024
College of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China.
Article Synopsis
- * Conducted over 20 years in Gaoyi, Hebei Province, the research utilized soil chemical analysis and high-throughput sequencing to assess changes in soil properties and the diversity of AOA and AOB communities.
- * Results indicated that soil nutrient levels and pH fluctuated with planting age, influencing AOA and AOB abundance and diversity, with specific nutrient factors identified as key drivers for each group’s community structure.
Genomic Insights of " Nitrosocaldaceae" Based on Nine New Metagenome-Assembled Genomes, Including " Nitrosothermus" Gen Nov. and Two New Species of " Nitrosocaldus".
Front Microbiol
January 2021
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
Article Synopsis
- "Nitrosocaldaceae" are a group of microorganisms found in hot springs and geothermally heated soils, playing a crucial role in the nitrogen cycle, but remain largely unstudied due to challenges in isolating them in pure form.
- This study utilized metagenomics to analyze samples from hot springs in India and China, leading to the identification of two new species within "Nitrosocaldus" and a new genus named "Nitrosothermus."
- Key metabolic genes for ammonia oxidation and thermal adaptation were found in the genomes, along with genes for urea degradation and vitamin biosynthesis, highlighting their unique adaptations to high-temperature environments.
Nitrogen and Oxygen Isotope Effects of Ammonia Oxidation by Thermophilic Thaumarchaeota from a Geothermal Water Stream.
Appl Environ Microbiol
August 2016
Laboratory of Ocean-Earth Life Evolution Research, Japan Agency for Marine-Earth Science and Technology, Yokosuka, Japan Department of Subsurface Geobiological Analysis and Research, Japan Agency for Marine-Earth Science and Technology, Yokosuka, Japan.
Unlabelled: Ammonia oxidation regulates the balance of reduced and oxidized nitrogen pools in nature. Although ammonia-oxidizing archaea have been recently recognized to often outnumber ammonia-oxidizing bacteria in various environments, the contribution of ammonia-oxidizing archaea is still uncertain due to difficulties in the in situ quantification of ammonia oxidation activity. Nitrogen and oxygen isotope ratios of nitrite (δ(15)NNO2- and δ(18)ONO2-, respectively) are geochemical tracers for evaluating the sources and the in situ rate of nitrite turnover determined from the activities of nitrification and denitrification; however, the isotope ratios of nitrite from archaeal ammonia oxidation have been characterized only for a few marine species.
View Article and Find Full Text PDFThermophilic prokaryotic communities inhabiting the biofilm and well water of a thermal karst system located in Budapest (Hungary).
Extremophiles
July 2015
Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C, Budapest, 1117, Hungary.
In this study, scanning electron microscopy (SEM) and 16S rRNA gene-based phylogenetic approach were applied to reveal the morphological structure and genetic diversity of thermophilic prokaryotic communities of a thermal karst well located in Budapest (Hungary). Bacterial and archaeal diversity of the well water (73.7 °C) and the biofilm developed on the inner surface of an outflow pipeline of the well were studied by molecular cloning method.
View Article and Find Full Text PDFA comprehensive census of microbial diversity in hot springs of Tengchong, Yunnan Province China using 16S rRNA gene pyrosequencing.
PLoS One
July 2013
State Key Laboratory of Biogeology and Environmental Geology and Institute of Earth Sciences, China University of Geosciences, Beijing, China.
The Rehai and Ruidian geothermal fields, located in Tengchong County, Yunnan Province, China, host a variety of geochemically distinct hot springs. In this study, we report a comprehensive, cultivation-independent census of microbial communities in 37 samples collected from these geothermal fields, encompassing sites ranging in temperature from 55.1 to 93.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!